Making Media + Gram stain of D. Radiodurans

On 4/7, I was shown how to make media. Media is to nourish the bacteria. They live in it and eat it as well.
The ingredients that were used to make media was:

  •  Tryptone, 1.25 grams
  • Yeast extract, 1.25 grams
  • Dextrose (Glucose), .25g 
  • Water, 250ml 
A flask was place on a stirring hot plate and it was turn to a medium speed and 100 ml of water was added. Add the ingredients one at a time. Lastly, add the remaining water, the 150 ml, which makes it a total of 250 ml of water. I was just shown how to make media, so I didn't do anything with it once it was made. 




Also,  I did a gram stain on D. Radiodurans. A gram stain is when you want to know whether a bacteria is gram-positive or gram-negative. Gram- positive bacteria have a thick layer of peptidoglycan in its cell wall, so its able to retain the crystal violet, making it look purple. After staining D. Radiodurans, it was found to be gram positive. 




Comments

  1. Ricardo M.April 12, 2018 at 9:36 PM

    That's a really cool picture of the gram stain. It almost looks like the bacteria where placed to grow in a pattern. It's a really neat picture. I've never heard of D Radiodirans. How easy is it to work with this bacteria? Is it like E. coli that's good to work with?

    ReplyDelete
  2. beccaonlineApril 21, 2018 at 9:07 PM

    I really like how you clarified what each thing you performed was and its purpose. Being new to biology, that is really helpful, so thank you! That's also a really cool gram stain picture, good job!

    ReplyDelete

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